Andrographolide promotes proliferative and osteogenic potentials of human placenta-derived mesenchymal stem cells through the activation of Wnt/β-catenin signaling

Abstract Introduction The in vitro expansion and differentiation of mesenchymal stem cells derived from bone marrow (BM-hMSCs) are considered as potential therapeutic tools for clinical applications tissue engineering regenerative medicine. However, invasive sampling reduction number proliferative capacity with age the major limitations BM-hMSCs. Recently, human placenta-derived MSCs (PL-hMSCs) obtained by a non-invasive procedure have attracted much interest. Attempts to increase PL-hMSCs would be an important paradigm Herein, we examined osteogenic effect andrographolide (AP) on PL-hMSCs. Methods Mesenchymal were isolated full-term normal placentas characterized before using. Cell cytotoxicity AP MTT BrdU assay, respectively. non-toxicity concentrations further assessed determined alkaline phosphatase (ALP) expression activity, alizarin red staining, osteoblast-specific gene expressions. Screening genes involved differentiation-related pathways modulated was explored NanoString nCounter analysis. Results generated this study met MSC criteria set International Society Cellular Therapy. non-cytotoxic up 10 μM. compound increased PL-hMSC proliferation concomitant increases Wnt/β-catenin level activity. It also enhanced association mRNA expression. Further, promoted formation structural protein level, osteocalcin, osteoblastic cells. Gene screening analysis showed upregulation related Wnt/β-catenin, TGFβ/BMP, SMAD, FGF signaling pathways. Conclusion We demonstrated, first time, role promoting proliferation, differentiation, osteoblast This suggests that may effective novel agent improvement cell-based therapy regeneration.